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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
05/12/2018 |
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Actualizado : |
26/08/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
RUBIO, L.; HERNÁNDEZ, L.; BENÍTEZ, M. J.; ARRUABARRENA, A.; RIVAS, F.; COLINA, R.; MAESO, D. |
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Afiliación : |
LETICIA PAOLA RUBIO CATTANI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LESTER HERNÁNDEZ RODRÍGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; MARÍA JOSÉ BENÍTEZ GALEANO, Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte (CENUR- Litoral Norte), Universidad de la República, Uruguay; ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS FERNANDO RIVAS GRELA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte (CENUR- Litoral Norte), Universidad de la República, Uruguay; DIEGO CESAR MAESO TOZZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Biological and molecular characterization of Uruguayan citrus tristeza virus field isolates. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Journal of Plant Pathology, 2019, volume 101, Issue 1, pages 97-105. |
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ISSN : |
1125-4653 // Online ISSN 2239-7264 |
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DOI : |
10.1007/s42161-018-0149-0 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 06 June 2018 // Accepted 29 July 2018 // First Online 13 August 2018 // Published 15 February 2019.
Acknowledgements: This research was funded by Instituto Nacional de Investigación Agropecuaria, Uruguay (project CT-06), through the National Program of Citrus, in collaboration with the Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte de la Universidad de la República.
Funding: This study was funded by Instituto Nacional de Investigación Agropecuaria, Uruguay (project CT-06). |
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Contenido : |
ABSTRACT.
Citrus tristeza virus (CTV) is the causal agent of the most important viral disease of citrus. Symptoms that may affect the productive potential of citrus plants are observed in Uruguayan orchards even though resistant rootstocks are used. CTVis fully eliminated in propagative materials by the National Sanitation and Certification Program, but since the virus and its vector are widespread in the country, the risk of infection in the field persists. In this situation, using mild CTV strains in a cross-protection program would be a useful alternative to attempt to increase yield and quality of the local citrus industry. To this aim, this study assessed the biological and molecular characteristics of 32 local CTV isolates. Bioassays were conducted in a greenhouse with controlled conditions. Each isolate was graft-inoculated on Mexican lime, sweet orange, sour orange and Duncan grapefruit indicator plants. Symptoms and their intensity were evaluated. Molecular characterization was carried out by RT-PCR amplification, using primers for the p25, p20 and p23 genes. PCR products were sequenced, nucleotide sequences were aligned with international reference strains and phylogenetic trees were constructed. Results of the biological and molecular analysis showed the prevalence of severe CTV isolates with a high genetic variability. Two out of 32 characterized isolates were selected as mild CTV isolates to be tested as candidates for future cross-protection experiments. The survey showed a complex scenario for the management of CTV in Uruguay.
© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2018 MenosABSTRACT.
Citrus tristeza virus (CTV) is the causal agent of the most important viral disease of citrus. Symptoms that may affect the productive potential of citrus plants are observed in Uruguayan orchards even though resistant rootstocks are used. CTVis fully eliminated in propagative materials by the National Sanitation and Certification Program, but since the virus and its vector are widespread in the country, the risk of infection in the field persists. In this situation, using mild CTV strains in a cross-protection program would be a useful alternative to attempt to increase yield and quality of the local citrus industry. To this aim, this study assessed the biological and molecular characteristics of 32 local CTV isolates. Bioassays were conducted in a greenhouse with controlled conditions. Each isolate was graft-inoculated on Mexican lime, sweet orange, sour orange and Duncan grapefruit indicator plants. Symptoms and their intensity were evaluated. Molecular characterization was carried out by RT-PCR amplification, using primers for the p25, p20 and p23 genes. PCR products were sequenced, nucleotide sequences were aligned with international reference strains and phylogenetic trees were constructed. Results of the biological and molecular analysis showed the prevalence of severe CTV isolates with a high genetic variability. Two out of 32 characterized isolates were selected as mild CTV isolates to be tested as candidates for future cross-protection experiments. The su... Presentar Todo |
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Palabras claves : |
BIOLOGICAL INDEXING; CITRUS TRISTEZA VIRUS (CTV); CLOSTEROVIRIDAE; CLOSTEROVIRUS; PHYLOGENETIC ANALYSIS. |
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Thesagro : |
CITRUS. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 03065naa a2200301 a 4500
001 1059343
005 2019-08-26
008 2019 bl uuuu u00u1 u #d
022 $a1125-4653 // Online ISSN 2239-7264
024 7 $a10.1007/s42161-018-0149-0$2DOI
100 1 $aRUBIO, L.
245 $aBiological and molecular characterization of Uruguayan citrus tristeza virus field isolates.$h[electronic resource]
260 $c2019
500 $aArticle history: Received 06 June 2018 // Accepted 29 July 2018 // First Online 13 August 2018 // Published 15 February 2019. Acknowledgements: This research was funded by Instituto Nacional de Investigación Agropecuaria, Uruguay (project CT-06), through the National Program of Citrus, in collaboration with the Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte de la Universidad de la República. Funding: This study was funded by Instituto Nacional de Investigación Agropecuaria, Uruguay (project CT-06).
520 $aABSTRACT. Citrus tristeza virus (CTV) is the causal agent of the most important viral disease of citrus. Symptoms that may affect the productive potential of citrus plants are observed in Uruguayan orchards even though resistant rootstocks are used. CTVis fully eliminated in propagative materials by the National Sanitation and Certification Program, but since the virus and its vector are widespread in the country, the risk of infection in the field persists. In this situation, using mild CTV strains in a cross-protection program would be a useful alternative to attempt to increase yield and quality of the local citrus industry. To this aim, this study assessed the biological and molecular characteristics of 32 local CTV isolates. Bioassays were conducted in a greenhouse with controlled conditions. Each isolate was graft-inoculated on Mexican lime, sweet orange, sour orange and Duncan grapefruit indicator plants. Symptoms and their intensity were evaluated. Molecular characterization was carried out by RT-PCR amplification, using primers for the p25, p20 and p23 genes. PCR products were sequenced, nucleotide sequences were aligned with international reference strains and phylogenetic trees were constructed. Results of the biological and molecular analysis showed the prevalence of severe CTV isolates with a high genetic variability. Two out of 32 characterized isolates were selected as mild CTV isolates to be tested as candidates for future cross-protection experiments. The survey showed a complex scenario for the management of CTV in Uruguay. © Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2018
650 $aCITRUS
653 $aBIOLOGICAL INDEXING
653 $aCITRUS TRISTEZA VIRUS (CTV)
653 $aCLOSTEROVIRIDAE
653 $aCLOSTEROVIRUS
653 $aPHYLOGENETIC ANALYSIS
700 1 $aHERNÁNDEZ, L.
700 1 $aBENÍTEZ, M. J.
700 1 $aARRUABARRENA, A.
700 1 $aRIVAS, F.
700 1 $aCOLINA, R.
700 1 $aMAESO, D.
773 $tJournal of Plant Pathology, 2019, volume 101, Issue 1, pages 97-105.
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Registro original : |
INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
21/10/2014 |
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Actualizado : |
25/09/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
B - 3 |
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Autor : |
MULVANEY, F.J.; MORRIS, S.T.; KENYON, P.R.; MOREL, P.C.H.; WEST, D.M.; VIÑOLES, C.; GLOVER, K.M.M. |
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Afiliación : |
CAROLINA VIÑOLES GIL, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Comparison between the reproductive performance of ewe hoggets and mature ewes following a progesterone-based oestrus synchronization protocol. |
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Fecha de publicación : |
2013 |
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Fuente / Imprenta : |
New Zealand Journal of Agricultural Research, 2013, v.56, no.4, p. 288-296. |
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Serie : |
0028-8233 |
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DOI : |
10.1080/00288233.2013.842176 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 27 May 2013 // Accepted 5 September 2013. |
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Contenido : |
ABSTRACT.
Two experiments were conducted to compare the reproductive performance of ewe hoggets and mature ewes. All ewes were synchronized by an intravaginal progesterone-releasing device and bred together over a 5-day period. In Experiment 1, of those joined with the ram, more mature ewes ovulated (99% vs 68%; P < 0.05) with a higher ovulation rate (1.82 vs 1.14; P < 0.05) than ewe hoggets. Mature ewes had a higher pregnancy rate (98% vs 47%; P < 0.05) and lower early pregnancy loss (13% vs 41%; P < 0.05) than ewe hoggets. In Experiment 2, the ovulation rate (1.84 vs 1.44; P < 0.05) and pregnancy rate (86% vs 7%; P < 0.05) was higher in mature ewes compared with ewe hoggets. Combined, these studies help to quantify the difference in reproductive performance during breeding and pregnancy between ewe hoggets and mature ewes, and help to direct further research.
© 2013 The Royal Society of New Zealand. |
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Thesagro : |
CORDEROS; REPRODUCCION ANIMAL. |
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Asunto categoría : |
L53 Fisiología Animal - Reproducción |
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Marc : |
LEADER 01779naa a2200253 a 4500
001 1051218
005 2019-09-25
008 2013 bl uuuu u00u1 u #d
024 7 $a10.1080/00288233.2013.842176$2DOI
100 1 $aMULVANEY, F.J.
245 $aComparison between the reproductive performance of ewe hoggets and mature ewes following a progesterone-based oestrus synchronization protocol.$h[electronic resource]
260 $c2013
490 $a0028-8233
500 $aArticle history: Received 27 May 2013 // Accepted 5 September 2013.
520 $aABSTRACT. Two experiments were conducted to compare the reproductive performance of ewe hoggets and mature ewes. All ewes were synchronized by an intravaginal progesterone-releasing device and bred together over a 5-day period. In Experiment 1, of those joined with the ram, more mature ewes ovulated (99% vs 68%; P < 0.05) with a higher ovulation rate (1.82 vs 1.14; P < 0.05) than ewe hoggets. Mature ewes had a higher pregnancy rate (98% vs 47%; P < 0.05) and lower early pregnancy loss (13% vs 41%; P < 0.05) than ewe hoggets. In Experiment 2, the ovulation rate (1.84 vs 1.44; P < 0.05) and pregnancy rate (86% vs 7%; P < 0.05) was higher in mature ewes compared with ewe hoggets. Combined, these studies help to quantify the difference in reproductive performance during breeding and pregnancy between ewe hoggets and mature ewes, and help to direct further research. © 2013 The Royal Society of New Zealand.
650 $aCORDEROS
650 $aREPRODUCCION ANIMAL
700 1 $aMORRIS, S.T.
700 1 $aKENYON, P.R.
700 1 $aMOREL, P.C.H.
700 1 $aWEST, D.M.
700 1 $aVIÑOLES, C.
700 1 $aGLOVER, K.M.M.
773 $tNew Zealand Journal of Agricultural Research, 2013$gv.56, no.4, p. 288-296.
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